Spirit Animo is the one hundred and first episode of Ben 10: Universal Road Trip.
When Dr. Animo loses his youth, he is on the verge of dying, so he uses the Gem of Souls, one of the Gems of Infinity that have to ability to grant the user immortality, and he vows to destroy Ben once and for all by using the ability of every spirit animal.
- Dr. Animo gains the Gem of Soul from the Planet Solaris to absorb every Spirit Animal to become a god, only to lose it to King Neron.
- The Gem of Souls (square shaped amythyst gem) has the ability to grant user immortality.
- Ben Tennyson
- Rook Blonko
- Helen Wheels
- Pierce Wheels
- Dr. Animo - loses his body after abusing the power of the Gem of Soul, only his head remains
- King Neron - has gained the 2nd Gem
- Dr. Animo dies, but regains his head and puts into a gorilla's body.